Extracellular matrix derived from dental pulp stem cells promotes osteogenic differentiation of gingival fibroblasts via Wnt and Hippo signaling pathway. Extracellular matrix derived from dental pulp stem cells promotes osteogenic differentiation of gingival fibroblasts via Wnt and Hippo signaling pathway

This study investigated the efficacy of decellularized ECM derived from human dental pulp stem cells (dECM_DPSCs) and gingival-derived mesenchymal stem cells (dECM_GSCs) as an inductive scaffold for osteogenic differentiation of GSCs. ECM was constructed from DPSCs and GSCs that were cultured in a growth medium supplemented with L-ascorbic acid (N-ECM) or osteogenic induction medium (OM-ECM). The gene expression profile was examined using a high-throughput RNA sequencing technique. Overall design: Comparative gene expression profiling analysis of RNA-seq data for GSCs reseeded on N and OM-dECM_DPSCs and GSCs