Grapevine vinification microbiome (must)

In the current work we aimed to identify the composition of the prokaryotic and eukaryotic vinification microbiome of two of the most representative vine cultivars of Peloponnese (Greece) Roditis and Sideritis. In this quest we sampled grapes from two well-defined terroirs per variety in the region of Agialieia. Grapes from each of terroir x variety combination were subjected to three different vinification processes: (a) spontaneous carried out by the autochthonous microbiota (b) spontaneous carried out by the autochthonous microbiota but controlled with preservatives added in commercial fermentations and (c) commercial inoculated with allochthonous inocula. Amplicon sequencing of the 16S rRNA gene and the ITS2 region in Illumina Hiseq was employed to identify the composition of the vine and vinification microbiome in the different terroirs and vinification treatments.