Analysis of gene expression in hindgut cells of EdnrB mutant embryonic day 14 mice

Neural Crest Cells (NCC) migrate through the gut during mouse development and are involved in mesenchymal patterning. However, in a Tcof hetrozygous mutant, NCC cells stop their migration at E14.5. Mutation of the EdnrB receptor also inhibits cell migration, but farther back. A FLEX conditional knockout of EdnrB only in NCC cells, also halts migration, however is still permissible for transplantated wt NCC cells. In order to better understand mechanisms of cell migration, the transcriptional environment of the gut was characterized by array analysis of Ednrb mutants. EdnrB mutant, EdnrB-FLEX(NCC-/-) mutant, and WT mouse cells of the hind gut at 14.5 days are compared using Affymetrix MOE430 version 2 for a total of 9 arrays.