Data underlying the publication: Biocatalytic PEI-PSS membranes through aqueous phase separation: influence of casting solution pH and operational temperature

The aim of this publication is to prepare polyelectrolyte complex membranes incorporated with lysozyme through a one-step and mild pH shift aqueous phase separation (APS) approach. The effects of lysozyme addition and casting solution pH on the membrane properties were studied. The biocatalytic membranes exhibited temperature dependent enzymatic activity. Moreover, the biocatalytic membranes demonstrate desirable enzymatic stability, maintaining 60% activity even after 60 days of storage. This study validates the potential of the water-based APS process as a straightforward approach for integrating enzymes into responsive biocatalytic membranes. The dataset contains the data presented in the publication: dynamic viscosity of the casting solutions, SEM images, pure water permeability, and the enzymatic activity data of all the membranes.

本研究旨在通过一步法温和pH调控水相分离（aqueous phase separation, APS）工艺，制备负载溶菌酶的聚电解质复合膜。本研究考察了溶菌酶添加量与制膜液pH对膜性能的影响。该生物催化膜呈现出温度依赖的酶促活性；此外，其具备优异的酶稳定性，储存60天后仍可维持60%的酶活性。本研究证实了水基APS工艺作为简便方法，可将酶整合至响应型生物催化膜中的应用潜力。本数据集包含本出版物中呈现的全部数据：制膜液的动态粘度、扫描电子显微镜（scanning electron microscope, SEM）图像、纯水渗透率以及所有膜的酶活性数据。