Data from:Investigation of urinary miRNA profile changes in amphotericin B-induced nephrotoxicity in C57BL/6 mouse, Sprague-Dawley rats and beagle dogs

MicroRNA (miRNAs) have been associated with drug-induced kidney injury
(DIKI). However, there are few reports on the utility of miRNAs, when
monitoring for nephrotoxicity across multiple species. The purpose of this
study was to assess the value of urinary miRNA profile changes as renal
safety biomarkers, when monitoring for kidney injury in investigative
toxicology studies. To this end, we evaluated urine miRNA expression
levels in response to amphotericin B (AmpB-induced nephrotoxicity in mice,
rats, and dogs. The results showed that 35 miRNAs were significantly
differentially expressed across the three species in response to the
induced renal injuries. Dogs showed the highest number of miRNAs with
significant changes. miR-205-5p and miR-31-5p were the most consistently
altered miRNA biomarkers across all three species. In rodents, these two
miRNAs were the most sensitive markers and showed comparable or better
sensitivities than the previously published urine protein biomarkers with
the same nephrotoxicant. In dogs, none of the upregulated miRNAs were as
sensitive as urine clusterin protein as observed in a previously published
study with AmpB. Taken together, these miRNAs could complement the more
established urinary protein biomarkers in monitoring DIKI in mice, rats,
and dogs. To our knowledge, this is the first report that demonstrates the
comparative utility of urinary miRNAs for the early detection of DIKI
across three nonclinical animal models.