Raphanus sativus Genome sequencing

Radish (Raphanus sativus) is an economically important crop grown for edible roots and leaves. It is a self-incompatible, outcrossing species to be difficult to produce homozygous lines to develop breeding populations. However, it can be overcome by doubled haploids using anther culture or isolated microspores providing the rapid production of homozygous lines for breeding. Thus, it would be useful to identify the radishes with the high reaeration rate from microspore culture. In the current study, 96 radish cultivars or germplasms were evaluated for high regeneration rates. Also, a single marker analysis (SMA) was applied to detect SNPs potentially associated with this trait using a genotype-by-sequencing (GBS) technology. The regeneration rate from microspore culture of 96 lines showed wide range from 0 to 269.5 percent. From the SMA, fifty-two markers were detected at least at the p-value of 0.001 and a total of 16 genes identified physically nearby; sixteen of them hit genes reported in various species as candidates for regeneration rates. This result could be used for clarifying genes underlying the traits and developing molecular markers associated with regeneration rates, beneficial to generating homozygous inbred lines.