Androgen receptor RIP-seq of LNCaP cells treated with androgen hormone. Homo sapiens

In this work we reported in a high-throughput way (RIP-seq) the RNAs associated with androgen receptor after treatment with androgen hormone. We used a large compilation of lincRNAs to describe the differences between lincRNAs associated to androgen receptor from those who are non-associated with androgen receptor. By integrating different data sources (DNA-seq Seq and CHIP-seq) was possible to describe transcription factors and histone marks diffrentially enriched at the promoter and vicinity of androgen associated lincRNA loci. Overall design: ​The LNCaP cell were cultivated by 6 hours in a medium containing 0.1 nM of synthetic androgen hormone (R1881). After this process RNA was immunopreciptated with anti-AR (2 biological replicates) or Anti-IgG (2 biological replicates).