Modularity and selection of nectar traits in the evolution of the selfing syndrome in Ipomoea lacunosa (Convolvulaceae)

Although the evolution of the selfing syndrome often involves reductions in floral size, pollen, and nectar, few studies of selfing syndrome divergence have examined nectar. We investigate whether nectar traits have evolved independently of other floral size traits in the selfing syndrome, whether nectar traits diverged due to drift or selection, and the extent to which quantitative trait locus (QTL) analyses predict genetic correlations.
We use F5 recombinant inbred lines (RILs) generated from a cross between Ipomoea cordatotriloba and I. lacunosa. We calculate genetic correlations to identify evolutionary modules, test whether trait divergence was due to selection, identify QTLs, and perform correlation analyses to evaluate how well QTL properties reflect genetic correlations.
Nectar and floral size traits form separate evolutionary modules. Selection has acted to reduce nectar traits in the selfing I. lacunosa. Genetic correlations predicted from QTL properties are consistent with ob..., We crossed Ipomoea cordatotriloba by Ipomoea lacunosa and generated a F2 mapping population; F2 individuals were selfed by single seed decent to the F5 generation. Nectaries were collected while phenotyping for nectar volume and nectar sugar concentration and stored in RNA later and in the -20 freezer. They were subsequently imaged using a Leica MZFLIII stereomicroscope set at 1X magnification and connected to a 4D HD digital microscope camera from Microscope Systems, Inc. (https://microscope-systems.com/)., , # Data from: Modularity and selection of nectar traits in the evolution of the selfing syndrome in *Ipomoea lacunosa* (Convolvulaceae)

[https://doi.org/10.5061/dryad.xpnvx0kgz](https://doi.org/10.5061/dryad.xpnvx0kgz)

## Nectary images

Images used for measuring/estimating nectary size are included in this dataset.

File naming:\
IMGxxxxx.JPG\
mIMGxxxxx.tif

where xxxxx is the number of the image, .JPG is the raw file, and .tif is the modified file with points selected using FIJI.

All scripts and additional information for converting the points into distances and associating the photos with the individual flower can be found in [https://github.com/itliao/IpomoeaNectarQTL/blob/main/Phenotypic_Datasets/NectarySize/](https://github.com/itliao/IpomoeaNectarQTL/blob/main/Phenotypic_Datasets/NectarySize/)