Additional file 1 of Docetaxel induced activation of GSDME pathway and pyroptosis enhance immune lethality in prostate cancer cells

Supplementary Material 1: Supplementary Fig 1. Docetaxel affects immune lymphocytes changes. A: Proportion of immune lymphocytes during non-small cell lung cancer and breast cancer chemotherapy cycle. B: The overlapping Venn diagrams of immune-related genes and docetaxel influencing genes. C: Relationship between related genes and immune cells in prostate tumors. n.s., no significance; *P < 0.05, ***P < 0.001,****P < 0.0001. Supplementary Fig 2. Docetaxel can induce a shift in the mode of death of prostate cancer cells. A: Schematic diagram of a mouse subcutaneous tumor model. B: Survival curves of mice in each group. Data represented as mean ± SD (n = 3). C: Experiment of Cell Plate Cloning under Drug Intervention; D: Quantitative Statistics of Colony Formation in Plate Cloning Experiment. Data represented as mean ± SD (n = 3). n.s., no significance; **P < 0.01, ***P < 0.001, ****P < 0.0001. Supplementary Fig 3. Docetaxel can induce a shift in the mode of death of prostate cancer cells. A: mRNA expression levels of GSDME in prostate cancer cell lines from HPA. B: protein expression levels of GSDME in prostate cancer cell lines. C: Changes of protein expression of Caspase1 and Caspase3 before and after docetaxel treatment D: mRNA expression levels of GSDME after GSDME overexpression. E: protein expression levels of GSDME after GSDME overexpression. F: CCK8 assay on the OD value in 96-well plates of the RM1 cells and DU145 cells after GSDME overexpression. G: Colony formation assay on colony numbers of the RM1 cells and DU145 cells after GSDME overexpression. Data represented as mean ± SD (n = 3). n.s., no significance;**P < 0.01, ***P < 0.001, ****P < 0.0001. Supplementary Fig 4. SKP2 ubiquitinates GSDME and promotes its degradation. A: protein expression levels of GSDME after adding different drugs. B: protein expression levels of GSDME in the condition of docetaxel and TFA. C: protein expression levels of GSDME in the condition of docetaxel and SKP2. D: The degree of molecular phosphorylation of GSDME under the intervention of IPL34. E: The degree of phosphorylation of the GSDME molecule after the mutation at the S252 position. Supplementary Fig 5. GSDME is closely related to immune environment. A: Relationship between GSDME segmentation and immune cells in pan-carcinoma. B: Enrichment analysis of GSDME overexpression data sets. C: Effect of differential expression of GSDME on immune cell expression. D: GSDME molecules are associated with immune cell infiltration from timer datesets. Supplementary Fig 6. GSDME can affect the immune invasion of prostate tumors. A: Different cells are subdistributed on chromosomes. B: Time sequence analysis of different cell subpopulations. C: Lymphocyte grouping affects functional changes D: Specific lymphocyte cluster enrichment function. E: Proportion of surviving tumor cells. Mechanism diagram Data represented as mean ± SD (n = 3). n.s, no significance, *P < 0.05,**P < 0.01. Supplementary Table 1. Oligonucleotides used for relative gene expression by qRT-PCR. Supplementary Table 2. The oligonucleotides of siRNA or shRNA. *The oligonucleotides from WeizhenBio, Shandong, China.