Transcriptomic analysis of the spiral ganglion from hearing and aminoglycoside deafened rats

We used RNAseq to assess transcriptomic changes in the spiral ganglion of rats deafened with kanamycin compared to hearing control rats to gain a better understanding of the deafening-induced changes that occur in the spiral ganglion. Transcriptomic profiles were obtained from whole spiral ganglion from hearing and deafened rats at P32 and P60. We observed significant changes in gene expression in not only deafened compared to hearing ganglia, but also from P32 to P60 in normal hearing animals. The most prominent change after deafening was an increase in immune response-related gene expression, indicating that immune response activation occurs in the deafened spiral ganglion.