Expression in donor monocyte derived cells

Monocyte differentiate into macrophages that display either pro-inflammatory or tolerogenic profile depending on the inflammatory condition of the intestine. We performed adoptive monocyte transfer to dissect the dynamics of monocyte to macropahges differentiation. Gene expression analysis of donor monocyte-derived cells recovered from non-inflamed and inflamed small intestine of recipient mice at day 1, 2 and 5 after transfer for in-depth investigation of monocyte development in the intestine. Transferred monocytes recovered and FACS-sorted from the small intenstine of recipient mice at different time points and used for gene expression analysis. Following sorting, cDNA was made immediately and preamplified with Multiplexed target enrichment (MTE) primers. Hybridization of pre-amplified DNA with target specific oligonucleotide probe pairs were performed using a nanostring technology.