A lysosomal hydrolase receptor, CPBF2, involve in invasion machinery of an enteric protozoan parasite Entamoeba histolytica

To know the role of lysosomal hydrolase receptors in virulecne of enteric protozoan parasite Entamoeba histolytica, eleven cysteine protease binding protein family (CPBF) proteins were specifically silenced and examined the effect on virulence-related phenotypes. Among them, CPBF2 gene silence caused defect in Matrigel invasion. To clarify transcriptomic difference in CPBF2 gene silenceing strain, RNA-seq analysis was conducted. Overall design: Examine RNA-seq analysis of mock transfected and CPBF2 gene silenceing strain. (n=3)