Additional file 4: of Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas

Results of the segmented regression analysis for assessing the stability of the antigen coupled beads per antigen. The breakpoints were estimated with the R-package ‘segmented’ and the ΔMFI-values obtained at Week (Wk) 4, Wk 8 and Wk16 were converted into percentages compared to the ΔMFI value obtained directly after coupling (Wk0).