Characterisation of ulvan from Ulva ohnoi and the immunomodulatory effect of ulvan on secreted cytokines from RAW264.7 cells

See chapter 5 of PhD thesis for complete details of data collection. The aims of this study were to elucidate the effect of the molecular weight of purified ulvan, extracted from Ulva ohnoi, on the inflammatory response of RAW264.7 murine macrophages. Chemical characterisation of ulvan from Ulva ohnoi through elemental, sulfate and ash quantification, size exclusion chromatography with multi-angle light scattering detector, constituent sugar analysis with high performance anion exchange chromatography with pulsed amperometric detection, and nuclear magnetic resonance spectroscopy. Immunmodulatory effect of ulvan assessed through the concentration of secreted cytokines from RAW264.7 cells in response to lipopolysaccharide and ulvan determined by ELISA.
The full methodologies will be available in the author's thesis (Chapter 5) from the Related Publications link below.