Integrated lncRNAs and mRNAs sequencing reveals lncRNAs regulatory network associated with lung injury in LPS-induced septic shock rats

Sepsis is associated with increased morbidity and mortality. Long non-coding RNAs (lncRNAs) have been associated with human diseases. Here, we used a microarray to analyze lncRNAs and mRNAs expression and functional network of lung injury in lipopolysaccharide (LPS)-induced septic shock rats. We established an LPS-induced septic shock model in rats. High-throughput sequencing was performed on the lung tissue to identify differentially expressed (DE) lncRNAs and mRNAs. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis was used to verify the microarray data. Gene Ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis were used to investigate the biological functions. Establishment of coding and non-coding co-expression (CNC) and competing endogenous RNA (ceRNA) were performed to study the molecular interactions of these molecules.