Exploring the binding motif in genome wide for p53, Pygo2 and H3K4-Me3 in mouse (Pten/Smad4 KO) PS cell line

Since our data support that Pygo2 function is p53 Wild type status dependent and the interaction of Pygo2 with H3K4-Me3 is needed for the c-Kit regulation by Pygo2, however, neither Pygo2 nor p53 bind at c-Kit promoter region. To find out the potential co-binding locia for Pygo2, p53 and H3K4-Me3, we performed a Cut&Run assay followed by Miseq Sequencing. Overall design: Examination of Pygo2, p53 and H3K4-Me3 binding locia in mouse genome, the fragmented input was used as control.