Mycobacterium tuberculosis strain:mnpk Transcriptome or Gene expression. Mycobacterium tuberculosis strain:mnpk

Next generation sequencing particularly RNA sequencing and analysis are playing a key role in investigating the complexity of gene regulation as well as expression and RNA-seq apply deep-sequencing technologies to find out the cDNA sequences and its expression. Here, we employed RNA-seq to explore the transcriptome of Mycobacterium tuberculosis under in vitro and in vivo culture conditions to investigate the M. tuberculosis pathogenesis isolated from Pakistan. Two profiles of In vitro and In vivo cultures (control media and artificial sputum media respectively) were generated following the removal of physical ribosomal RNA. We thoroughly illustrated the RNA sequence data under two culture conditions (In vitro and In vivo) and found the differential gene expression of two culture conditions.