Single-nucleus multiome profiling of islet cells from beta-cell specific inducible Xbp1s OE mice. Mus musculus strain:C57

This study explores the dynamic transcriptional and chromatin accessibility changes induced by beta-cell-specific overexpression of Xbp1s in pancreatic islet cells using a Dox-inducible transgenic mouse model. To capture time-dependent regulatory changes, single-nucleus RNA sequencing (snRNA-seq) and single-nucleus ATAC sequencing (snATAC-seq) were performed on islet cells isolated from transgenic mice at multiple time points: before Dox induction (0h), after 24h and 72h of Dox treatment, and after 72h of Dox treatment followed by 48h of Dox withdrawal. All data were generated using the 10x Genomics Chromium Single Cell Multiome platform and Illumina sequencing. This dataset provides insights into the reversible regulatory mechanisms of Xbp1s overexpression in pancreatic beta-cells.