(A) Gel electrophoresis of NGS-derived PCR amplicons shows large deletions of POLR2J4 in clones #6 and #7 samples. (B) POLR2J4 indel rate. M indicates the normal cell line. #6 indicates a cell line wi
Forty-seven isolated Jurkat clones were screened for HDR-edited TRIM5 gene by PCR using a mutated TRIM5 specific primer. The presence of a 157 bp band indicates that the primer could bind and that the
