Summary of Ae. aegypti OX4312 injections for ΦC31-RMCE.

*All G1 transformants from OX4312 injections into OX4372A resulted in incomplete cassette exchange due to only a single pair of attachment sites recombining. Embryos of this partially recombined line, called ‘OX4372A[OX4312]-incomplete’ were injected with additional ΦC31 recombinase mRNA resulting in a second recombination step in G1 progeny (Efficiency = 18.5%).Recombination events (R) are defined as the number of transgenic pools. Efficiency = minimum calculated integration efficiency.Summary of Ae. aegypti OX4312 injections for ΦC31-RMCE.