Ibuprofen Solubility in Eutectic Solvents Formed by Choline-Chloride and Polyols

Ibuprofen (IBU) is a widely known nonsteroidal anti-inflammatory drug used to treat various diseases, but due to its poor solubility, its bioavailability and formulation options are limited. The aim of this study was to evaluate the solubility of ibuprofen in various choline chloride (ChCl) and polyol (sorbitol (SOR), xylitol (XYL), mannitol (MAN), sucrose (SUC), isomalt (ISOM) based eutectic solvents. The use of eutectic systems represents an environmentally friendly alternative to conventional organic solvents and may enhance the solubility and bioavailability of poorly soluble compounds.
Method
Samples were prepared by the shake-flask method, mixing 500 mg of ChCl with different polyols in corresponding ratios, and adding 100 mg of ibuprofen, with varying water content (0-25% v/v). The mixtures were incubated for 24 hours in Eppendorf (DNA LoBind Tube 1.5mL) tubes and mixed using magnetic stirring (10mm stir bars) at 60-80 °C depending on polyol. After incubation, the samples were centrifuged (Hermle LaborTechnik GmbH - Z 160 M Microlitre Centrifuge) for 25 minutes at maximum rotation speed, and 50 mg aliquots of the lower layer were diluted in 5 mL acetonitrile (ACN).
Ibuprofen solubility was determined using a Thermo Scientific Vanquish HPLC system. The mobile phase consisted of 55% acetonitrile (ACN) and 45% of a 2% formic acid aqueous solution. The analysis was performed at a flow rate of 1 mL/min on a C18 Zorbax column at temperature 45 oC. The ibuprofen amount was measurement at 222 nm. Each sample was prepared in duplicate, and results were expressed as mg of ibuprofen per 1 g of solvent.
Data
The dataset contains the experimental data and results of the solubility of ibuprofen in different choline chloride–polyol deep eutectic solvents with different water content (0-25%), along with representative HPLC chromatogram pictures. The data were processed using Chromeleon 7.3.1 Chromatography Data System (Thermo Fisher Scientific).