Sequencing of matched pair samples (diagnosis and relapse) in human B-cell acute lymphoblastic leukemia cells (ALL)

The experiment focused on how B-cells respond transcriptionally to drug treatment in acute lymphocytic leukemia (ALL). We obtained B-cell samples from a patient at diagnosis (LAX7) and after the development of resistance (LAX7R) following a standard 3-week chemotherapy regimen (vincristine, dexamethasone, L-asparaginase and doxorubicin). The resistance sample possessed a KRASG12V mutation not present at diagnosis. Cells obtained from the patient were directly engrafted into NOD/SCIDγc-/- mice, and later frozen as stocks. Genome-wide profiling of total RNA transcript levels in human B-cell acute lymphoblastic leukemia cells (ALL)