The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat

Male SHRs and normotensive Wistar-Kyoto(WKY) rats were purchased from Vital River (Vital River Laboratory Animal Technology Co., Ltd, Beijing, China). Briefly, eightteen SHRs aged seven to eight weeks old were randomly assigned to one of three groups: water group (drinking tap water), water containing 333 mg/L or 1g/L TMAO group (TMAO, product number: T1362, Tokyo Chemical Industry, Japan). Six WKY rats of the same age were used as normotensive controls. In this study, WKY rats (n=6), SHR (n=6), and SHR treated with 1g/L TMAO (n=6) were uesd for experiments. Overall design: Visceral white adipose tissue (vWAT) was sampled from the abdominal cavity. RNA of visceral white adipose tissue was isolated using the TRIzol reagent/chloroform isolation method. SMART technology (Clontech) was used to construct the RNA-seq libraries. RNA sequencing was performed using a illumine Nova-Seq 6000 System with the technical support of the Guangzhou Epibiotek Co., Ltd. The GENCODE database was used to annotate mRNAs. RNA reads were counted using featureCounts (http://bioinf.wehi.edu.au/featureCounts/). The FPKM method was employed to normalize gene expression counts for the sequence.