Data_Sheet_1_Identification of photoperiod-induced specific miRNAs in the adrenal glands of Sunite sheep (Ovis aries).ZIP

In seasonal estrus, it is well known that melatonin-regulated biorhythm plays a key role. Some studies indicate that the adrenal gland plays an important role in reproduction in mammals, but the molecular mechanism is not clear. This study used an artificially controlled light photoperiod model, combined with RNA-seq technology and bioinformatics analysis, to analyze the messenger RNA (mRNA) and microRNA (miRNA) of ewe (Sunite) adrenal glands under different photoperiod treatments. After identification, the key candidate genes GRHL2, CENPF, FGF16 and SLC25A30 that photoperiod affects reproduction were confirmed. The miRNAs (oar-miR-544-3p, oar-miR-411b-5p, oar-miR-376e-3p, oar-miR-376d, oar-miR-376b-3p, oar-miR-376a-3p) were specifically expressed in the adrenal gland. The candidate mRNA-miRNA pairs (e.g., SLC25A30 coagulated by novel miRNA554, novel miRNA555 and novel miRNA559) may affect seasonal estrus. In summary, we constructed relation network of the mRNAs and miRNAs of sheep adrenal glands using RNA sequencing and bioinformatics analysis, thereby, providing a valuable genetic variation resource for sheep genome research, which will contribute to the study of complex traits in sheep.

在季节性发情期，众所周知，由褪黑素调节的生物钟发挥着至关重要的作用。一些研究表明，肾上腺在哺乳动物的繁殖过程中扮演着重要的角色，但其分子机制尚不明确。本研究采用人工控制的光周期模型，结合RNA测序技术和生物信息学分析，对绵羊（Sunite）肾上腺在不同光周期处理下的信使RNA（mRNA）和微RNA（miRNA）进行了分析。经鉴定，受光周期影响，对繁殖至关重要的关键候选基因GRHL2、CENPF、FGF16和SLC25A30得到确认。特定的miRNAs（如oar-miR-544-3p、oar-miR-411b-5p、oar-miR-376e-3p、oar-miR-376d、oar-miR-376b-5p、oar-miR-376a-5p）在肾上腺中特异性表达。候选mRNA-miRNA对（例如，由新型miRNA554、新型miRNA555和新型miRNA559凝集的SLC25A30）可能影响季节性发情。总之，本研究通过RNA测序和生物信息学分析构建了绵羊肾上腺mRNA和miRNA的关系网络，从而为绵羊基因组研究提供了宝贵的遗传变异资源，将对绵羊复杂性状的研究做出贡献。