Epigenetic application of ATAC-seq based on Tn5 transposase purification technology. Epigenetic application of ATAC-seq based on Tn5 transposase purification technology

We report a set of rapid, efficient and low-cost methods for ATAC-seq library construction and data analysis, realized large-scale and rapid sequencing. These methods can provide a reference for the study of epigenetic regulation of gene expression. Overall design: We propose reliable approaches for purifying the hyperactive Tn5 transposase by Chitin Magnetic bead sorting. Double-stranded DNA of J76 cells and 293T cells were digested and subjected to tagmentation as test samples with Tn5 transposase, and libraries were established and sequenced. Sequencing data was then analyzed for peak calling, GO enrichment and motif analysis.