Activity of TDH3 cis-regulatory variants in YPG (glycerol)

Quantify effects on expression of mutations and polymorphisms in the TDH3 promoter upon growth in YPG (3% glycerol).
Conclusion:
Comparing the effects of mutations and polymorphisms provided no evidence that selection acted on TDH3 cis-regulatory activity in glycerol. However, cis-regulatory mutations had different impact in glycerol and glucose (data collected in glucose for the same strains are in experiment FR-FCM-ZZBN).
Notes:
Samples were grown in YPG and run on a BD Accuri C6 flow cytometer in SC-Arg liquid media with 3% glycerol as carbon source. Each plate contains 20 genetically identical samples at fixed positions used to correct for batch effects.