Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Fibroblasts_after_Dex]

The goal of this study was to understand the role of fibroblasts in thymic regeneration following dexamethasone-induced acute involution. For this purpose we treated WT mice with dexamethasone, sorted fibroblasts from their thymi 4 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA Seq. Overall design: Young male WT mice were treated with dexamethasone 4 days/18 hours before sacrifice, and then fibroblasts were isolated and mRNA profiles were generated by deep sequencing using the MARSseq method. 3-4 replicates were used. The control was untreated age-matched WT mice.