Supplementary data: Description of two new subspecies of Bacillus cabrialesii.

Strain TSO2T, a plant growth-promoting rhizobacteria and biological control agent, was isolated from wheat rhizosphere sampled from the Yaqui Valley in Mexico. The strain was identified using a polyphasic approach. Based on its analysis of the full-length 16S rRNA gene, strain TSO2T was assigned to the genus<em> Bacillus</em>, which was supported by morphological and metabolic traits, such as Gram-positive staining, rod shape, spore formation, strictly aerobic metabolism, catalase-positive activity, starch, and casein hydrolysis, reduction of nitrate to nitrite, growth in presence of lysozyme and 2% NaCl, citrate utilization, growth at pH 6.0, acid production from glucose and indole production from tryptophan. Additionally, strain TSO2T possesses swarming motility, presenting a featureless mat pattern that can cover the whole petri dish. The whole-genome phylogenetic relationship analysis elucidated that strain TSO2T is closely related to <em>Bacillus cabrialesii</em> TE3T. The maximum values for average nucleotide identity (ANI) and <em>in silico</em> DNA–DNA hybridization from the genome-to-genome distance calculator (GGDC) were 97% and 73.4%, respectively, related to <em>Bacillus cabrialesii</em> TE3T, where both ANI and GGDC values were barely above the species delimitation threshold, but below the subspecies limit. Also, strain TSO2T showed the ability to produce a fatty acid (C18:0) that is not present in closely related <em>Bacillus</em> species. These results provide evidence that strain TSO2T is a novel subspecies of the species <em>Bacillus cabrialesii</em>, for which the name <em>Bacillus cabrialesii</em> subsp. <em>mobilis</em> subsp. nov. is proposed. The type strain of <em>Bacillus cabrialesii</em> subsp. <em>mobilis</em> subsp. nov. is TSO2T (CM-CNRG TB52T = LBPCV TSO2T). The description of this novel subspecies automatically creates the subspecies <em>Bacillus cabrialesii</em> subsp. <em>cabrialesii </em>subsp. nov. for which the type strain is TE3T (CM-CNRG TB54T= CCStamb A1T).