Establishment and characterization of prostate cancer organoids [array]

Prostate cancer translational research has been hampered by the lack of comprehensive and tractable models that represent the genomic landscape of clinical disease. Metastatic castrate-resistant prostate cancer (mCRPC) patient derived xenografts (PDXs) recapitulate the genetic and phenotypic diversity of the disease. We sought to establish a representative, preclinical platform of PDX-derived organoids that is experimentally facile for high throughput and mechanistic analysis. To investigate the effects of organoid establishment on clonal selection and drift, we assessed somatic copy number alteration in DNA derived from PDX and multiple generations of organoid from the same individual. The raw data is being submitted to dbGaP study phs001587 due to patient privacy concerns. NIH grant(s): ZIA BC011782 Mechanisms of Pathogenesis in Patient Derived Organoid Models of Prostate Cancer NATIONAL CANCER INSTITUTE KATHLEEN SIEBENLIST ZIA BC011679 Mechanisms Driving Evolution of Aggressive Prostate Cancer NATIONAL CANCER INSTITUTE ADAM SOWALSKY