Pilot study of DNA methylation, molecular aging markers and measures of health and well-being in aging

Genome wide DNA methylation assays was conducted using the Illumina Infinium MethylationEPIC BeadArray technology (Methyl850K chip) that allows genome-wide DNA methylation analysis of 866,836 CpG sites. Samples included 11 psychiatric cases and 12 healthy controls balanced by age decade and sex. Bisulfite conversion, or bisulfite treatment, whole genome amplification (WGA), hybridization and single base extension were carried out to get labeled nucleotide using standard protocol. Dual color (Cy3 and Cy5) staining was used for allowing the nucleotide to be detected by the iSCAN reader and is converted to genotype during the analysis.