ZmbZIP4-ChIPseq-input-1.wiggle.tar.gz
收藏Mendeley Data2024-01-31 更新2024-06-27 收录
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https://figshare.com/articles/ZmbZIP4-ChIPseq-input-1_wiggle_tar_gz/6225806/1
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The construct pCAMBIA1302-35S::ZmbZIP4-GFP was used for transformation of Maize inbred Qi319 young embryo via a gene gun (Bio-Rad). Stable transgenetic maize and the monoclonal antibody of GFP were used for ChIP-seq assay. The production and confirmation of transgenic lines were accomplished as previously described. The details of ChIP were as described by Kaufmann et al. (Kaufmann and Al, 2010). Sequencing platform: Illumina Hiseq 4000. Aligner and parameter: Clean Parameter “soap_mm_gz -p 4 -v 5 -s 35 -m 0 -x 600”. Peak caller and parameter: Peak Calling Parameter “macs14 -g 411831487 -p 1e-5 -w --space 50 -m 10, 30”.
创建时间:
2024-01-31



