ACSS2 Regulates HIF-2α Degradation through the E3-Ubiquitin Ligase MUL1 in Clear Cell Renal Cell Carcinoma. ACSS2 Regulates HIF-2α Degradation through the E3-Ubiquitin Ligase MUL1 in Clear Cell Renal Cell Carcinoma

ACSS2 is a key contributor to the acetyl-CoA pool and has a role in supporting biosynthetic processes, as well as acetylation. To investigate the role of ACSS2 in regulating chromatin accessibility in ccRCC, we performed ATAC-seq on 786-O cells treated with DMSO or ACSS2i for 24 hours. Overall design: We treated 786-O cells with DMSO or 5 µM ACSS2i for 24 hours and performed ATAC-seq to assess changes to accessible regions.