Candidate pro-death and pro-survival genes identified by RNAi and cell viability assay.

Gene symbols, CG numbers (column 1), and functions (column 5) are from Flybase [58]. The indicated Ortholog symbols (column 6) are from the HUGO Gene Nomenclature Committee (HGNC). P-values in columns 2 and 3 were calculated by comparing the WST-1 reading (A450–A650) from RNAi of the gene of interest to the WST-1 reading from RNAi of the human (Hs) negative control (NM_138278). Ecdysone dependent means that the observed viability effects of RNAi depended on the presence of ecdysone. Italicized and bolded P-values in column 3 indicate that these genes showed a pro-survival effect in the absence of ecdysone. Each RNAi treatment had three replicates and the assay was conducted at least twice. Superscript symbols a, b, and c indicate that these genes were identified in other related RNAi screens; a = Boutrous et al., 2004 [49]; b = Björklund et al., 2006 [47]; c = Bettencourt Dias et al., 2004 [57].