Function of Ets1 in regulating macrophage polarization and adipose inflammation

To investigate the cooperative function Ets1 in macrophage, we generats Ets1 knockout mice.We then performed gene expression profiling analysis in macrophages seperated from either control or ko mice. We also performed Ets1 ChIP-seq, using M1 and M2 macrophages seperated from wild type C57 bone marrow, for mechanism study. Overall design: Transcription sequencing (RNA-seq) in macrophages seperated from either Ets1 null or control mice, were perfomed. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for Ets1 in M1 and M2 macrophages, were perfomed.