Effect of sodium butyrate on mt-DNA content, citrate synthase activity and mitofusin I relative expression.

(a)Mitochondrial DNA quantification (mt-DNA/nDNA). Total DNA (cytosolic and mitochondrial) was extracted from control and NaB treated cells and NADH-dehydrogenase subunit I (a gene coded by the mitochondrial genome) content was estimated by real-time PCR analysis. Actin DNA (a nuclear coded gene) was used to normalize DNA amounts. Values represent mean ± SEM; N = 4.(b)Specific citrate synthase activity (µmol x mg−1 x min−1) present in mitochondrial fraction of H460 cells treated with 10 mM NaB for 24 h. Values represent mean ± SEM; N = 5.(c)Mitofusin I mRNA relative expression. Actin was used to normalize cDNA amounts. Values represent mean ± SEM; N = 3, *P