Gene expression profiles of irradiated WT and CREPT deleted intestinal epithelium

Intestinal stem cells (ISCs) residing in the crypts are critical for the continual self-renewal and rapid recovery of intestinal epithelium. The regulatory mechanism of ISCs is not fully understood. Here we report that CREPT, a recently identified tumor-promoting gene, is preferably expressed in the crypts, where the ISCs reside, but not in the villi. The Lgr5+ ISCs have much higher CREPT protein level than Lgr5- cells. To explore the function of CREPTduring regeneration, we isolated irradiated WT and CREPT deleted intestines (Vil-CREPTKO) to perform Next generation sequencing. To verify the role of CREPT in intestine, we knock out CREPT in intestinal epithelium by introducing villin-cre into CREPT flox/flox mice. We applied X-ray irradiation and allowed the regeneration of intestinal villus and crypts. Total RNAs were extracted from irradiated intestines, and sequenced by BGISEQ-500.