5'DFUR treatment in MCF7. Effect at 4 hours. Homo sapiens

MCF7 cells were treated during 90 min with 5'DFUR 250 uM in presence or absence of NBTI 100nM. The effect was analyzed 4 hours after the beginning of treatment. RNA from two independent experiments was isolated and hybridization was performed in duplicate in all cases, using reciprocal labelling. Keywords: 5'DFUR effect. Time 4h Overall design: MCF7 cells were treated during 90 min with 5’-DFUR 250 mM either in the presence or the absence of NBTI 100 nM. After 4 hours from the beginning of the treatment cells were trypsinized and total RNA was isolated and amplified. To ensure the reproducibility of the technique cDNA hybridization was performed in duplicate in all cases, using reciprocal labelling. Moreover, RNA from two independent experiments was isolated, thus, four microarray were hybridized for each condition.