Transcriptomic analysis of the nickel response in roots and shoots in the nickel hyperaccumulator Leucocroton havanensis (Euphorbiaceae) endemic from Cuba.

The goal of this study was to produce a reference transcriptome for the nickel hyperaccumulator Leucocroton havanensis endemic from Cuba and use this transcriptome as a reference to identify genes responding to the presence or the absence of nickel in both roots and shoots of this species To perform this analysis, Leucocroton havanensis plantlets were grown in vitro on a medium without or with nickel for 6 weeks after germination. Roots and shoots were then collected collected separatly to extract RNA. cDNA were then sequenced by Illumina paired-end sequencing. One repetition per sample was used in this study .