Transcriptional response in the heart after low-dose gamma

We report whole transcriptome sequencing of the intraventricular septum of mouse heart 2 and 6 weeks after 25 Gy ionizing radiation. The objective of this experiment was to identify genes and pathways differentially regulated by ionizing radiation in the heart. Image-guided isocentric gamma irradiation was delivered to experimental groups under isoflurane anesthesia in a single, 25 Gy dose over approximately 15 minutes. Control group animals were experimental group littermates which received anesthesia and CT imaging but no radiation. RNA was isolated from intraventricular septum using TRIzol. Whole transcriptome profiles of mouse heart intraventricular septum after 25 Gy single dose ionizing radiation. Three groups of n=6 per group: a normal reference control (ctrl), 2 weeks post irradiation (2wk), and 6 weeks post irradiation (6wk)