Defining stationary phase transcriptomes of Campylobacter jejuni cell morphotypes

Filamentation, the transition from rod to filamentous cell morphology, has been identified as a response to stressful conditions in many bacterial species. Campylobacter jejuni exhibits filamentation upon entry to stationary phase. This study examines the cell mophotype specific transciptomes (RNAseq) of C. jejuni. Cell morphotypes were rapidly seperated by filtration from stationary phase Campylobacter jejuni cultures before RNA extraction. Ribosomal RNAs were removed and directional RNAseq perfomed (Illumina HiSeq 2500) to examine cell morphotype-specific transcription at stationary phase compared to short spiral forms harvested from exponential phase growth.