Development of gene expression signatures for lung cancer cells interaction with human monocyte-derived macrophages

To further investigate the lung cenr progression, we have employed gene expression profiling as a discovery platform to identify genes with the potential involved in MDM interaction . Human peripheral monoctyte cells (PBMCs) from lung cancer patients were collected. PBMCs were treated with GM-CSF and differentiated to MDM. MDM cells were cocultured with or without lung cancer cells for 24h. Total RNAs were extracted from H827 cancer cells.