Mus musculus Raw sequence reads. Mus musculus

Background: Ischemia/reperfusion (I/R)-mediated myocardial infarction (MI) is a major pathological factor implicated in the progression of ischemic heart disease but the key factors dys-regulated during in the I/R-MI remains unclear, especially those essential non-coding factors required for cardiovascular development.Methods: The murine I/R-MI model and RNA sequencing (RNA-seq) was used to identify key lncRNAs after myocardial infarction. qRT-PCR was used to validate expression in cardiac muscle tissues and myocardial cells. The role of Gm18840 in HL-1 cell growth was determined by flow cytometry experiments in vitro. The full-length of Gm18840 was identified by using Rapid amplification of cDNA ends (RACE) assay. Subcellular distribution of Gm18840 was examined by nuclear/cytoplasmic RNA fractionation and qRT-PCR. RNA pull-down and RNA Immunoprecipitation (RIP)-qPCR assays were performed to identify Gm18840-interacted proteins. The ChIRP-seq (Chromatin Isolation by RNA purification) was used for identifying the genome-wide binding of Gm18840 on chromatin. The regulatory activity of Gm18840 in transcriptional regulation were examined by luciferase reporter assay and qRT- PCR.Results: Gm18840 was upregulated post the myocardial infarction in both in vivo and in vitro I/R-MI models. Gm18840 was defined to be 1471 nt in length and localized in both cytoplasm and nucleus of HL-1 cells. Functional studies shown that knockdown of Gm18840 inhibited the apoptosis of HL-1 cells. Gm18840 directly interact with the histones, including the H2B, highlighting a potential function on transcriptional regulation. Further ChIRP-seq and RNA-seq analyses shown that the Gm18840 directly bound at the cis-regulatory regions of genes involved in development processes, such as Junb, Rras2 and Bcl3.