Mechanisms Coordinating Ribosomal Protein Gene Transcription in Response to Stress

In this study, we elucidate the common logic of the RPGs regulatory network by evaluating both the architecture and activity of promoters under conditions of stress or modulation of TF levels, and we identified the proteins regulating the activity of promoters lacking Rap1 binding, thus demonstrating that RPG co-regulation requires the complementary action of two different mechanisms involving both Ifh1 and Sfp1. Transcriptional responses were measured by RNA Polymerase II binding upon depletion of transcription factors in budding yeast. Sfp1 and Ifh1 binding on RPG promoters was measured by ChEC-Seq.