This project aims to sequence, in a whole-genome scale, five derivates of the thermophilic bacteria Thermus thermophilus HB27 in order to detect SNPs and compare the differences between mutants and its parental strains, quantify plasmid copy number of each strain and study the coverage of lectures along the genome.

Here, we present the whole genome sequencing of five Thermus thermophilus HB27 strains used to study the physiological role of Ttago and TthPrimpol in DNA interference. The starting wild type strain is T. thermophilus HB27 (ATCC BAA-163/DSM7039), which was used to generate the strain HB27 ?primpol, a markerless deletion mutant of TT_C0656 loci, generated by cre – lox system. Additionally, a second HB27 ?primpol (called HB27 ?primpol Clone 2) was isolated from an independent colony of the same parental strain and using the same cre – lox system.On the other hand, we generated from the strain T. thermophilus HB27EC, the mutant HB27EC ?ago, a markerless deletion mutant of TT_P0026 loci, obtained by homologous recombination and further loss of the antibiotic resistant cassette. The last mutant strain is HB27EC ?ago?primpol, a double mutant employing T. thermophilus HB27EC ?ago as parental strain and using cre – lox system to delete TT_C0656 loci.HB27, HB27 ?primpol, HB27EC ?ago and HB27EC ?ago?primpol were transformed prior sequencing with plasmid pMoTK_3A (5) to quantify plasmid copy number. HB27 ?primpol Clone 2 was not transformed with the plasmid.