Datasets for "Balancing barcoding and genomics: gDNA Quality in Insect Vouchers after HotSHOT DNA extraction"

The excel sheet "Dataset_BalancingBarcodingAndGenomics" contains information for each specimen used in the experiments reported in "Balancing barcoding and genomics: gDNA Quality in Insect Vouchers after HotSHOT DNA extraction", mainly DNA Integrity Numbers, DNA concentrations of genomic DNA (gDNA) and HotSHOT DNA (hsDNA) extractions, as well as summaries of the Tapestation reports for each specimen.
The excel sheet "HotSHOT_protocols_review_21012026" contains an overview of peer‐reviewed studies published from 2006 to 2026 employing the HotSHOT DNA extraction protocol. For each reference, the column ‘HotSHOT protocol used’ indicates whether the original protocol (Standard: 95°C for 30 min) or a modified version was applied. This classification is based on the methodological descriptions provided by the authors, which are reproduced verbatim in the ‘Verbatim protocol’ column.