RNA-seq of mouse sperm samples from different treatment groups

The goal of this project was to explore the effect of NH3 and/or H2S effect on the mouse sperm RNA expression and the transgenerational effects. RNA-seq of mouse sperm samples from different groups (NH3 and or H2S treatment) in different generations was done to search for transgenerational effects on RNA expression. RNA-seq of mouse sperm samples from different groups in different generations to search for transgenerational effects of NH3 and/or H2S treatment. First, three weeks of age ICR male mice were treated with NH3 and H2S for 5 weeks (F0 mice). Then the mice (F0) mated with normal ICR female mice. After delivery, F1 male mice were raised normally (without any treatments). At the 8 weeks of age, F1 male mice mated with normal female mice. The F2 male mice were raised normally without any treatment. The semen was collected for F0, F1, F2 mice for the RNA-seq to search for the transgenerational effects of NH3, H2S.