Single cell RNA sequencing of post-traumatic brain injury day 4 ipsilateral brain leukocytes in C57BL/6 adult male mice

The goals of this project include 1) determining the cellular immune response induced by acute traumatic brain injury in vivo with a focus on microglia and monocyte/macrophages, and 2) correlate immune responses with improved function post-TBI in Ccr2-/- TBI mice, which lack monocyte infiltration into the injured brain and are resistant to brain damage, compared to WT TBI mice. Overall design: Leukocytes were isolated from ipsilateral brain tissues from four groups of mice: wildtype (WT) TBI mice (n=3), Ccr2-/- TBI mice (n=3), WT normal (n=3), Ccr2-/- normal (n=2). CD45+ Ly6G- LIVE cells were sorted by flow cytometry, and cells were processed for single-cell RNA sequencing using the 10X Genomics platform v3 chemistry.