ChIP-seq of PAX8 and H3K27ac in Kuramochi ovarian cancer cells

This study aims to characterize the genomic binding landscape of the transcription factor PAX8 and the distribution of the active histone mark H3K27ac in the human high-grade serous ovarian cancer (HGSC) cell line Kuramochi. PAX8 is a lineage-defining transcription factor that plays a central role in ovarian cancer biology and transcriptional regulation.Chromatin immunoprecipitation sequencing (ChIP-seq) datasets were generated from the human high-grade serous ovarian cancer (HGSC) cell line Kuramochi. ChIP-seq was performed using antibodies against the transcription factor PAX8 and the histone modification H3K27ac, a marker of active enhancers and promoters. Chromatin from formaldehyde-crosslinked cells was sonicated and immunoprecipitated, followed by high-throughput sequencing on an Illumina HiSeq 2500 platform.Sequencing reads were aligned to the human reference genome (GRCh38) using BWA, and peaks were identified using MACS2 with an FDR threshold of 0.05. Each ChIP experiment was performed in three independent biological replicates. These data provide genome-wide maps of PAX8 binding sites and H3K27ac-marked regulatory elements in Kuramochi ovarian cancer cells.