Transcriptomic changes in the pituitary when pituitary enhancer of the Nr5a1 gene is deleted

NR5A1 is expressed in the pituitary gonadotropes and regulates their functional differentiation. We have previously identified a pituitary-specific enhancer region located in the 6th intron of the Nr5a1 gene. In this study, deletion of the pituitary enhancer by genome editing abolished the expression of NR5A1 in the pituitary gland, confirming the functional importance of the enhancer. Transcriptomic changes in the enhancer-deleted mouse pituitary were revealed in both males and females. Overall design: We collected pituitaries from control mice (n=4 for male and female) and the mice in which pituitary enhancer of the Nr5a1 gene is deleted (n=4 for male and n=3 for female).