Mating environments mediate the evolution of behavioral isolation during ecological speciation

The evolution of behavioral isolation is often the first step towards speciation. While past studies show that behavioral isolation will sometimes evolve as a by-product of divergent ecological selection, we lack a more nuanced understanding of factors that may promote or hamper its evolution. The environment in which mating occurs may be important in mediating whether behavioral isolation evolves for two reasons. Ecological speciation could occur as a direct outcome of different sexual interactions being favored in different mating environments. Alternatively, mating environments may vary in the constraint they impose on traits underlying mating interactions, such that populations evolving in a ‘constraining’ mating environment would be less likely to evolve behavioral isolation than populations evolving in a less constraining mating environment. In the latter, mating environment is not the direct cause of behavioral isolation but rather permits its evolution only if other drivers are ..., Data collection is described in the associated manuscript by Barerra et al. (2024) in Evolution Letters., , # Mating environments mediate the evolution of behavioral isolation during ecological speciation

Data collection is described in the associated paper: Barerra et al. (2024) in Evolution Letters. There were three assays (experiments) performed. R code and accompanying data files are provided separately for each. Files with 'RawData' in the name are not called by the R script, but are provided for completeness as they include additional information not included in processed data files.

## Description of the data and file structure

**assay1_RawData**\
Rows are individual replicate mating trials. Columns are as follows:\
A-D: Mating environments (simple vs. complex) and larval environments (salt vs corn) of first (cols A and B) and second (cols C and D) populations in a given mating trial pair.\
E-H: color (R = red, G= Green) of males and females from the first and second population\
I: indicates whether the male and female from a given population were the same or different color.\
J: ...